Asked by Morgan Bradley on Jul 02, 2024
Verified
Researchers are beginning the process of DNA cloning. They have chromosomal DNA, a plasmid cloning vector, restriction enzymes, and DNA ligase. What else is needed in order to perform DNA cloning? Why is this needed?
Plasmid Cloning Vector
A small, circular DNA molecule used in genetic engineering to artificially replicate foreign genetic material within a host organism.
DNA Cloning
The process of making multiple, identical copies of a specific segment of DNA, often used in genetic engineering and research.
DNA Ligase
A substance that assists in the fusion of DNA strands by enabling the development of a phosphodiester bond.
- Understand the prerequisites and steps involved in DNA cloning.
Verified Answer
AM
Andres Molina Navarro7 days ago
Final Answer :
The researcher needs bacteria. Reproducing bacteria are needed to amplify the copies of the DNA of interest. The recombinant plasmid is inserted into a host bacterial cell. When the cell reproduces, it copies the plasmid along with its chromosome. Each descendant cell receives a plasmid.
Learning Objectives
- Understand the prerequisites and steps involved in DNA cloning.