Asked by Chris Adams on Sep 23, 2024
Verified
The isoelectric point is important in ________.
A) the enzymatic resolution of amino acids
B) electrophoresis
C) determination of the C-terminal amino acid
D) determination of the N-terminal amino acid
E) the ninhydrin test
Isoelectric Point
The pH at which a molecule, especially an amino acid or protein, carries no net electric charge, making it electrically neutral.
Enzymatic Resolution
A process using enzymes to separate enantiomers of a racemic mixture based on their reactivity.
Electrophoresis
A laboratory technique used to separate charged molecules, such as DNA, RNA, or proteins, based on their size and charge by applying an electric field.
- Understand the role of isoelectric points in techniques like electrophoresis and in determining amino acid properties.
Verified Answer
DC
Destiny Chaplin4 days ago
Final Answer :
B
Explanation :
The isoelectric point is the pH at which a protein has a net charge of zero and it plays a key role in determining how a protein will behave in electrophoresis. Proteins will migrate towards the electrode of opposite charge until they reach their isoelectric point, at which point they will stop moving. Therefore, electrophoretic separation of proteins is based largely on differences in their isoelectric points.
Learning Objectives
- Understand the role of isoelectric points in techniques like electrophoresis and in determining amino acid properties.