Asked by Jenna Cadorette on Sep 23, 2024
Verified
The reagent used in the determination of the N-terminal amino acid residue of a peptide is ________.
A) carboxypeptidase
B) phenyl isothiocyanate
C) dicyclohexylcarbodiimide
D) ethyl chloroformate
E) peroxyformic acid
Phenyl Isothiocyanate
A chemical reagent used in the Edman degradation method for sequencing amino acids in peptides.
N-terminal
The N-terminal refers to the start or initiating end of a polypeptide or protein, characterized by the presence of an amino group (-NH2).
Amino Acid
A molecule that combines to form proteins, containing a basic amino group, an acidic carboxyl group, and a unique side chain, serving as the building block of proteins.
- Familiarize oneself with the approaches and agents used in peptide bond synthesis and structural analysis.
Verified Answer
TL
The life of La’Nell5 days ago
Final Answer :
B
Explanation :
Phenyl isothiocyanate reacts with the N-terminal amino acid residue of a peptide to form a phenylthiocarbamoyl derivative, which can be identified and quantified. The other reagents listed are used for various other steps in peptide analysis.
Learning Objectives
- Familiarize oneself with the approaches and agents used in peptide bond synthesis and structural analysis.