Asked by Meaghan Crowley on Jun 04, 2024
Verified
What is the substance required to cleave the vector DNA during recombinant DNA technology?
A) restriction enzymes
B) DNA ligase
C) plasmids
D) DNA polymerase
E) DNA helicase
Restriction Enzymes
Enzymes that cut DNA at specific recognition sequences, widely used in molecular biology.
Recombinant DNA Technology
A set of biotechnological techniques used to manipulate DNA sequences and produce new genetic combinations for various applications.
Vector DNA
Vector DNA refers to a molecule of DNA, such as a plasmid or virus, used to transfer genetic material into a cell in genetic engineering processes.
- Recognize and elucidate the instruments and procedures utilized in biotechnology, including PCR, restriction enzymes, DNA ligase, and RNA interference.
Verified Answer
DN
David NdothyaJun 08, 2024
Final Answer :
A
Explanation :
Restriction enzymes are used to cleave the vector DNA (usually a plasmid) and the source DNA (which contains the gene of interest) at specific recognition sites, allowing them to be joined together through complementary base pairing and ligation with DNA ligase. DNA polymerase and DNA helicase are not directly involved in the cleavage process.
Learning Objectives
- Recognize and elucidate the instruments and procedures utilized in biotechnology, including PCR, restriction enzymes, DNA ligase, and RNA interference.