Asked by Nathaly Hernandez on Sep 23, 2024

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What is the practical limit on the use of carboxypeptidase in providing sequence information?

A) two residues on the C-terminal end
B) two residues on the N-terminal end
C) three residues on the N-terminal end
D) three residues on the C-terminal end
E) six residues on the N-terminal end

Carboxypeptidase

An enzyme that catalyzes the hydrolysis of the carboxyl-terminal (C-terminal) peptide bond in a protein or peptide.

Sequence Information

Refers to the order of units (such as nucleotides in nucleic acids or amino acids in proteins) within a macromolecule, critical for determining its function and properties.

  • Describe the process of deriving amino acid sequence data from proteins and peptides.
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SJ
Samantha jones4 days ago
Final Answer :
A
Explanation :
Carboxypeptidase specifically cleaves amino acids from the C-terminal end of a protein or peptide chain, but its practical use for sequencing is generally limited to identifying only the last one or two amino acids due to its decreasing efficiency as it moves further from the end.